Anticorps Polyclonal de lapin anti-Piezo1 (extracellular domain)

• Phare
• Validé par KD/KO

Piezo1 (extracellular domain) Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 28511-1-AP

Synonymes

FAM38A, KIAA0233, Mib, PIEZO1, PIEZO1 (extracellular domain)

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Galerie de données de validation

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  WB analysis of HeLa using 28511-1-AP

  WB result of Piezo1 (extracellular domain) antibody (28511-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and Piezo1 (extracellular domain) knockout HeLa cells.

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  WB analysis using 28511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28511-1-AP (Piezo1 (extracellular domain) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28511-1-AP (Piezo1 (extracellular domain) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 28511-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28511-1-AP (PIEZO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28511-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28511-1-AP (PIEZO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules hTERT-RPE1
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

28511-1-AP cible Piezo1 (extracellular domain) dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Piezo1 (extracellular domain) Protéine recombinante Ag29858
• Nom complet: family with sequence similarity 38, member A
• Masse moléculaire calculée: 286 kDa
• Poids moléculaire observé: 280-300 kDa
• Numéro d’acquisition GenBank: BC008073
• Symbole du gène: FAM38A
• Identification du gène (NCBI): 9780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells which influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as PIEZO1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Fam38A also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in gene encoding PIEZO1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1.

Publications

Species	Application	Title
mouse	WB,IF	Glia; Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes; Authors - Yue Wan ; KD Validated
human	WB,IF,IP	FASEB J; Mechanical stretch activates piezo1 in caveolae of alveolar type I cells to trigger ATP release and paracrine stimulation of surfactant secretion from alveolar type II cells.; Authors - Kathrin Diem ; KD Validated
human	WB	JCI Insight; Mechanosensitive Piezo1 channels mediate renal fibrosis.; Authors - Xiaoduo Zhao
human	WB,CoIP	Heliyon; The function of Piezo1 in hepatoblastoma metastasis and its potential transduction mechanism; Authors - Xiaoshuo Ye ; KD Validated
mouse	WB,IF	Neuron; Microglial Piezo1 senses Aβ fibril stiffness to restrict Alzheimer's disease; Authors - Jin Hu