Anticorps Polyclonal de lapin anti-PIAS4

PIAS4 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

N° de cat : 18469-1-AP

Synonymes

PIASy, PIAS-gamma, PIASG, PIAS gamma, EC:2.3.2.27

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Galerie de données de validation

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  WB analysis of HL-60 using 18469-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18469-1-AP (PIAS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 18469-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18469-1-AP (PIAS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18469-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18469-1-AP (PIAS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 18469-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PIAS4 antibody (18469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 18469-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PIAS4 (18469-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules Jurkat, cellules K-562
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18469-1-AP cible PIAS4 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIAS4 Protéine recombinante Ag13316
• Nom complet: protein inhibitor of activated STAT, 4
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57-70 kDa
• Numéro d’acquisition GenBank: BC010047
• Symbole du gène: PIAS4
• Identification du gène (NCBI): 51588
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members(PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).