Anticorps Polyclonal de lapin anti-PIAS4

• Phare
• Validé par KD/KO

PIAS4 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 14242-1-AP

Synonymes

E3 SUMO protein ligase PIAS4, PIAS gamma, PIAS4, Piasg, PIASY, ZMIZ6

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Galerie de données de validation

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  WB analysis of K-562 using 14242-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 14242-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 14242-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14242-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HL-60, tissu ovarien de souris, tissu pancréatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IF: See 3 publications below

Informations sur le produit

14242-1-AP cible PIAS4 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIAS4 Protéine recombinante Ag5498
• Nom complet: protein inhibitor of activated STAT, 4
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57-70 kDa
• Numéro d’acquisition GenBank: BC029874
• Symbole du gène: PIAS4
• Identification du gène (NCBI): 51588
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members (PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	Nat Commun; Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA.; Authors - Emmanuelle Despras ; KD Validated
mouse	WB,IF	PLoS Biol; DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program.; Authors - Yao-Long Yan ; KD Validated
mouse	WB	PLoS One; Protein inhibitor of activated STAT, PIASy regulates α-smooth muscle actin expression by interacting with E12 in mesangial cells.; Authors - Torikoshi Kazuo K ; KD Validated
human	WB	Front Nutr; Hengshun Aromatic Vinegar Ameliorates Vascular Endothelial Injury via Regulating PKCζ-Mediated Oxidative Stress and Apoptosis.; Authors - Xin Li
human	WB	Nat Commun; Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy; Authors - Shouheng Jin ; KD Validated