Anticorps Polyclonal de lapin anti-PIAS2

PIAS2 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 16074-1-AP

Synonymes

ARIP3, DAB2 interacting protein, DIP, E3 SUMO protein ligase PIAS2, miz, MIZ1, PIAS NY protein, PIAS2, PIASX, PIASX ALPHA, PIASX BETA, SIZ2, ZMIZ4

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Galerie de données de validation

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  WB analysis using 16074-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16074-1-AP (PIAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16074-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16074-1-AP (PIAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16074-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16074-1-AP (PIAS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 16074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 16074-1-AP (PIAS2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules MCF-7
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

16074-1-AP cible PIAS2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIAS2 Protéine recombinante Ag9032
• Nom complet: protein inhibitor of activated STAT, 2
• Masse moléculaire calculée: 63 kDa, 69 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC015190
• Symbole du gène: PIAS2
• Identification du gène (NCBI): 9063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du
human	WB,IF	J Mol Med (Berl); Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.; Authors - Yuhong Zhang