Anticorps Polyclonal de lapin anti-PI4KB

• Phare
• Validé par KD/KO

PI4KB Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 13247-1-AP

Synonymes

PI4 kinase beta, PI4K BETA, pi4K92, PI4KB, PI4Kbeta, PI4KIIIbeta, PIK4CB, PtdIns 4 kinase beta

---

Galerie de données de validation

• 

  WB analysis of K-562 using 13247-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13247-1-AP (PI4KB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH-3T3 cells (RNAi) using 13247-1-AP

  WB result of anti- PI4Kb (13247-1-AP) with NIH-3T3 cells (RNAi).

• 

  WB analysis of Jurkat using 13247-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13247-1-AP (PI4KB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 13247-1-AP

  IP result of anti-PI4KB (IP:13247-1-AP, 4ug; Detection:13247-1-AP 1:300) with K-562 cells lysate 3600ug.

• 

  IHC staining of human liver using 13247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13247-1-AP (PI4KB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13247-1-AP (PI4KB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C6 using 13247-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using PI4KB antibody (13247-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules NIH-3T3 (ARNi)
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules C6,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 2 publications below

Informations sur le produit

13247-1-AP cible PI4KB dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PI4KB Protéine recombinante Ag3989
• Nom complet: phosphatidylinositol 4-kinase, catalytic, beta
• Masse moléculaire calculée: 801 aa, 90 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC040300
• Symbole du gène: PI4KB
• Identification du gène (NCBI): 5298
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PI4KB(phosphatidylinositol 4-kinase beta) is also named as PIK4CB, NPIK, PI4K92 and belongs to the PI3/PI4-kinase family. It may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation and involved in Golgi-to-plasma membrane trafficking. PI4KB is a type III enzyme that is sensitive to wortmannin(PMID:9020160). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human,mouse	WB	Cell Res; Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity; Authors - Ying Gao
human	IF	EMBO J; Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.; Authors - Zhengming Wu
human	WB	PLoS Pathog; Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.; Authors - Tao Wang
human		Virol Sin; Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo; Authors - Shangrui Guo
human	WB,IF	J Virol; N-terminal acetyltransferase 6 facilitates enterovirus 71 replication by regulating PI4KB expression and replication organelle biogenesis; Authors - Hang Yang
rat	WB	Cereb Cortex; Neonatal exposure to sevoflurane induces adolescent neurobehavioral dysfunction by interfering with hippocampal glycerophoslipid metabolism in rats.; Authors - Yonghai Wang