Anticorps Polyclonal de lapin anti-PHKB

• Phare
• Validé par KD/KO

PHKB Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 13400-1-AP

Synonymes

PHKB, phosphorylase kinase, beta

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 13400-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13400-1-AP (PHKB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13400-1-AP (PHKB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 13400-1-AP

  IP result of anti-PHKB (IP:13400-1-AP, 4ug; Detection:13400-1-AP 1:500) with mouse heart tissue lysate 1680 ug.

• 

  IHC staining of mouse liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13400-1-AP (PHKB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13400-1-AP (PHKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13400-1-AP (PHKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP, tissu cardiaque de rat, tissu cardiaque de souris, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cardiaque de souris,
• Résultats positifs en IHC: tissu hépatique de souris, tissu de muscle squelettique de souris, tissu de muscle squelettique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

13400-1-AP cible PHKB dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHKB Protéine recombinante Ag4240
• Nom complet: phosphorylase kinase, beta
• Masse moléculaire calculée: 1086 aa, 124 kDa
• Poids moléculaire observé: 124 kDa
• Numéro d’acquisition GenBank: BC033657
• Symbole du gène: PHKB
• Identification du gène (NCBI): 5257
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHKB gene encodes phosphorylase kinase subunit beta involved in glycan biosynthesis and glycogen metabolism. PHKB activity is regulated by phosphorylation of various serine residues, and catalyzes the phosphorylation of serine in certain substrates, including troponin I. Phosphorylase kinase (PhK) complex, composed of alpha, beta, gamma, and delta subunits, stimulates energy production from glycogen in the cascade activation of glycogenolysis. Its large homologous alpha and beta subunits regulate the activity of the catalytic gamma subunit. Defects in PHKB are the cause of glycogen storage disease type 9B (GSD9B) also known as phosphorylase kinase deficiency of liver and muscle (PKD), characterized by hepathomegaly, only slightly elevated transaminases and plasma lipids, clinical improvement with increasing age, and remarkably no clinical muscle involvement.

Publications

Species	Application	Title
mouse	IP, WB	Mol Endocrinol; 20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway.; Authors - Lai Guangrui G
mouse	WB	iScience; Loss of RNA-Binding Protein Sfpq Causes Long-Gene Transcriptopathy in Skeletal Muscle and Severe Muscle Mass Reduction with Metabolic Myopathy.; Authors - Motoyasu Hosokawa
human	WB,IHC	J Cancer Res Clin Oncol; Phosphorylase kinase β affects colorectal cancer cell growth and represents a novel prognostic biomarker.; Authors - Guanghui Wang ; KD Validated