Anticorps Polyclonal de lapin anti-PHIP

PHIP Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 20933-1-AP

Synonymes

ndrp, PH interacting protein, PHIP

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Galerie de données de validation

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  WB analysis using 20933-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 20933-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 20933-1-AP

  IP result of anti-PHIP (IP:20933-1-AP, 4ug; Detection:20933-1-AP 1:3000) with A375 cells lysate 3600ug.

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  IF Staining of MCF-7 using 20933-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 20933-1-AP (PHIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A375, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules A375
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

20933-1-AP cible PHIP dans les applications de WB, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHIP Protéine recombinante Ag14968
• Nom complet: pleckstrin homology domain interacting protein
• Masse moléculaire calculée: 1821 aa, 207 kDa
• Poids moléculaire observé: 207 kDa
• Numéro d’acquisition GenBank: BC008909
• Symbole du gène: PHIP
• Identification du gène (NCBI): 55023
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.

Publications

Species	Application	Title
human	WB,IF	Cell Metab; Exome Sequencing Identifies Genes and Gene Sets Contributing to Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC Transcription.; Authors - Gaëlle Marenne
human	ChIP	Cell Commun Signal; RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter; Authors - Jae-Hyun Jo
human	WB	BMC Cancer; LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter; Authors - Ganglin Ren