Anticorps Monoclonal anti-Prohibitin

Prohibitin Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

4D3G5

N° de cat : 60092-1-Ig

Synonymes

Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, PHB, PHB1, prohibitin

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Galerie de données de validation

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  WB analysis using 60092-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60092-1-Ig (Prohibitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60092-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60092-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 60092-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 60092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 60092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 60092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60092-1-Ig (Prohibitin Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 60092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60092-1-Ig(PHB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 60092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60092-1-Ig(PHB antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 60092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60092-1-Ig (Prohibitin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 60092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 60092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 60092-1-Ig

  Immunofluorescent analysis of Hela cells, using PHB antibody 60092-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules HeLa, tissu cardiaque de porc, tissu cardiaque de rat, tissu placentaire humain
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

60092-1-Ig cible Prohibitin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Prohibitin Protéine recombinante Ag1240
• Nom complet: prohibitin
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC013401
• Symbole du gène: PHB
• Identification du gène (NCBI): 5245
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).

Publications

Species	Application	Title
human,mouse	WB	Int J Chron Obstruct Pulmon Dis; Prohibitin Protects Pulmonary Microvascular Endothelial Cells Against Cigarette Smoke Extract-Induced Cell Apoptosis and Inflammation.; Authors - Yating Peng
human	IF	Oncotarget; Prohibitin, relocated to the front ends, can control the migration directionality of colorectal cancer cells.; Authors - Li-Li Ma