Anticorps Polyclonal de lapin anti-Prohibitin

• Phare
• Validé par KD/KO

Prohibitin Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 10787-1-AP

Synonymes

Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, PHB, PHB1, prohibitin

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Galerie de données de validation

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  WB analysis using 10787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10787-1-AP (Prohibitin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10787-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10787-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10787-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10787-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10787-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10787-1-AP (Prohibitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10787-1-AP (Prohibitin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10787-1-AP (PHB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10787-1-AP (PHB antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10787-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10787-1-AP (Prohibitin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of H9C2 using 10787-1-AP

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Prohibitin antibody (10787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10787-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Prohibitin antibody (10787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules Raji, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu hépatique humain, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules H9C2, cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10787-1-AP cible Prohibitin dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Prohibitin Protéine recombinante Ag1240
• Nom complet: prohibitin
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC013401
• Symbole du gène: Prohibitin
• Identification du gène (NCBI): 5245
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).

Publications

Species	Application	Title
human	WB	Cell; Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts.; Authors - Bei-Bei Chu
human	WB	Autophagy; PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.; Authors - Chaojun Yan
mouse	WB	Cell Death Differ; Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.; Authors - H Li
human	WB	Hum Mol Genet; Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.; Authors - McCray Brett A BA
mouse	WB	Cell Mol Gastroenterol Hepatol; DJ-1 deficiency in hepatocytes improves liver ischemia-reperfusion injury by enhancing mitophagy.; Authors - Min Xu
human	WB	Elife; Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.; Authors - Sven Dennerlein

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (02-24-2021)

HEK293T whole cell lysate. Membrane was blocked in 5% BSA for 1 hour then incubated overnight at 4 degrees with the primary antibody (1:1000)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

Prohibitin Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:10787-1-AP)