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Anticorps Polyclonal de lapin anti-PR

PR Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 19980-1-AP

Synonymes

NR3C3, PGR, PR, progesterone receptor

Galerie de données de validation

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PR Polyclonal antibody (19980-1-AP)

    IF Staining of MCF-7 using 19980-1-AP

    Immunofluorescent analysis of MCF-7 cells using 19980-1-AP (PR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PR Polyclonal antibody (19980-1-AP)

    IF Staining of MCF-7 using 19980-1-AP

    Immunofluorescent analysis of MCF-7 cells using 19980-1-AP (PR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Applications testées

Résultats positifs en IHCtissu de cancer du sein humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules MCF-7

Dilution recommandée

ApplicationDilution
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

19980-1-AP cible PR dans les applications de IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet progesterone receptor
Masse moléculaire calculée 933 aa, 99 kDa
Numéro d’acquisition GenBankNM_000926
Symbole du gène Progesterone Receptor
Identification du gène (NCBI) 5241
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.