Anticorps Polyclonal de lapin anti-Placental Growth Factor

• Phare
• Validé par KD/KO

Placental Growth Factor Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 10642-1-AP

Synonymes

D12S1900, PGF, PGFL, PIGF, Placenta growth factor, placental growth factor, PLGF, PlGF 2, SHGC 10760

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Galerie de données de validation

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  WB analysis using 10642-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10642-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10642-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human meningioma using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human meningioma using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu placentaire de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu de cancer du sein humain, tissu de méningiome humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• IHC: See 8 publications below
• IF: See 9 publications below

Informations sur le produit

10642-1-AP cible Placental Growth Factor dans les applications de IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Placental Growth Factor Protéine recombinante Ag0969
• Nom complet: placental growth factor
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC007789
• Symbole du gène: PGF
• Identification du gène (NCBI): 5228
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Placenta growth factor (PLGF) is a homodimeric glycoprotein, 46-50 kDa in size, belonging to the vascular endothelial growth factor (VEGF) sub-family. It plays an important role in pathological VEGF-driven angiogenesis. PLGF is expressed not only in placental cells but also in colon and mammary carcinomas. It acts as a primary inflammatory instigator of atherosclerotic plaque instability and thus may be useful as a risk-predicting biomarker in patients with acute coronary syndromes (ACS).

Publications

Species	Application	Title
rat	IF	Brain.; Global gene expression profiling of somatic motor neuron populations with different vulnerability identify molecules and pathways of degeneration and protection.; Authors - Hedlund E
mouse		Biomaterials; Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.; Authors - Yudong Song ; KD Validated
mouse	IHC,IF	Biomed Pharmacother; HIF-1α inhibitor YC-1 suppresses triple-negative breast cancer growth and angiogenesis by targeting PlGF/VEGFR1-induced macrophage polarization; Authors - Yan Li
human	IHC	Cancers (Basel); Occurrence of Fibrotic Tumor Vessels in Grade I Meningiomas Is Strongly Associated with Vessel Density, Expression of VEGF, PlGF, IGFBP-3 and Tumor Recurrence.; Authors - Katharina Hess
mouse	IF	Diabetes Obes Metab; Polarized macrophages promote gestational beta cell growth through extracellular signal-regulated kinase 5 signalling.; Authors - Yinan Jiang
mouse	IF	Int J Mol Sci; Alzheimer's Amyloid β Peptide Induces Angiogenesis in an Alzheimer's Disease Model Mouse through Placental Growth Factor and Angiopoietin 2 Expressions; Authors - Abdullah Md Sheikh