Anticorps Polyclonal de lapin anti-PGD

• Phare
• Validé par KD/KO

PGD Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 14718-1-AP

Synonymes

6PGD, PGD, PGDH, phosphogluconate dehydrogenase

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Galerie de données de validation

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  WB analysis using 14718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14718-1-AP (PGD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14718-1-AP

  IP result of anti-PGD (IP:14718-1-AP, 4ug; Detection:14718-1-AP 1:4000) with HepG2 cells lysate 1925 ug.

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  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14718-1-AP

  Immunofluorescent analysis of HepG2 cells using 14718-1-AP (PGD antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IHC: See 4 publications below

Informations sur le produit

14718-1-AP cible PGD dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PGD Protéine recombinante Ag6444
• Nom complet: phosphogluconate dehydrogenase
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 53 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC000368
• Symbole du gène: PGD
• Identification du gène (NCBI): 5226
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)

Publications

Species	Application	Title
human	WB	Nat Commun; Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.; Authors - Xiaoyu Ma
mouse	WB	Clin Transl Med; SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching.; Authors - Tiantong Ou
human	IHC	J Invest Dermatol; Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.; Authors - Zhichao Gu
human	WB	Biochem Pharmacol; PMID: 32535103; Authors - Qi Feng
mouse	WB	iScience; The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.; Authors - Tirthankar Sinha
human	WB,IHC	Carcinogenesis; YTH domain family 2 promotes lung cancer cell growth by facilitating 6-phosphogluconate dehydrogenase mRNA translation.; Authors - Hao Sheng ; KD Validated