Anticorps Polyclonal de lapin anti-PGCP

PGCP Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16601-1-AP

Synonymes

CPQ, LCH1, Lysosomal dipeptidase, PGCP, plasma glutamate carboxypeptidase

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Galerie de données de validation

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  WB analysis of L02 using 16601-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16601-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16601-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 16601-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16601-1-AP

  IP result of anti-PGCP (IP:16601-1-AP, 4ug; Detection:16601-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IF Staining of L02 using 16601-1-AP

  Immunofluorescent analysis of L02 cells using 16601-1-AP (PGCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02, tissu placentaire humain, tissu plasmatique humain, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IF/ICC: cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

16601-1-AP cible PGCP dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PGCP Protéine recombinante Ag9770
• Nom complet: plasma glutamate carboxypeptidase
• Masse moléculaire calculée: 472 aa, 52 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC020689
• Symbole du gène: PGCP
• Identification du gène (NCBI): 10404
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGCP(plasma glutamate carboxypeptidase) is also named as LCH1,CPQ and belongs to the peptidase M28 family. It may play an important role in the hydrolysis of circulating peptides. It catalyzes more efficiently the hydrolysis of dipeptides with unsubstituted terminals into amino acids. It is a proteinase that acts on the unsubstituted N- and C-termini of dipeptides. It has been suggested that this PGCP is involved in the release of thyroxine(PMID:20951214). Human PGCP is a metallopeptidase with five potential glycosylation sites, whose activity depends on dimerizationand it can be detected a band of 65-72kd in FRTL-5 cells which is pro-PGCP(PMID:22127294). It can be N-glycosylated(PMID:10206990). It has the pro-form of 60-65 kDa and mature form of 55 kDa(PMID:20951214).

Publications

Species	Application	Title
human,rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li