Anticorps Polyclonal de lapin anti-PFTK1

PFTK1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 21612-1-AP

Synonymes

CDK14, hPFTAIRE1, KIAA0834, PFTAIRE protein kinase 1, PFTAIRE1, PFTK1

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Galerie de données de validation

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  WB analysis of HeLa using 21612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 21612-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21612-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21612-1-AP (PFTK1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21612-1-AP (PFTK1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

21612-1-AP cible PFTK1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PFTK1 Protéine recombinante Ag16259
• Nom complet: PFTAIRE protein kinase 1
• Masse moléculaire calculée: 469 aa, 53 kDa
• Poids moléculaire observé: 48-53 kDa
• Numéro d’acquisition GenBank: BC136477
• Symbole du gène: PFTK1
• Identification du gène (NCBI): 5218
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PFTK1 is a Cdc2-related protein kinase, also named as PFTAIRE1, CDK14 and belongs to the CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It acts as a cyclin-dependent kinases that regulates cell cycle progression and cell proliferation. The PFTK1 protein shows high homology with mouse Pftaire1, a Cdc2-related protein expressed primarily in the postnatal and adult nervous system(PMID:9202329). The protein contains 2 predicted nuclear localization signals that the N-terminal nuclear localization signals do not play a role in vivo(PMID:11313143). It has 3 isoforms produced by alternative splicing. This antibody is specific to PFTK1.

Publications

Species	Application	Title
human	WB	Mol Ther Oncolytics; Acquired chemoresistance can lead to increased resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus; Authors - Dakota W. Goad
mouse	WB, IHC, IF	Toxicol Lett; CDK14 expression is down-regulated by cigarette smoke in vivo and in vitro.; Authors - Daniel Pollack
human	WB	World Neurosurg; MiR-205 Inhibits Sporadic Vestibular Schwannoma Cell Proliferation by Targeting Cyclin-Dependent Kinase 14.; Authors - Xiaoling Yin
human	WB,IHC	Biomed Pharmacother; MiR-542-3p, a microRNA targeting CDK14, suppresses cell proliferation, invasiveness, and tumorigenesis of epithelial ovarian cancer.; Authors - Jingwei Li