Anticorps Polyclonal de lapin anti-PFKP

• Phare
• Validé par KD/KO

PFKP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 13389-1-AP

Synonymes

6 phosphofructokinase type C, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase

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Galerie de données de validation

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  WB analysis using 13389-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13389-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13389-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13389-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13389-1-AP

  IP result of anti-PFKP (IP:13389-1-AP, 4ug; Detection:13389-1-AP 1:4000) with Jurkat cells lysate 2280 ug.

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  IP experiment of mouse heart using 13389-1-AP

  IP result of anti-PFKP (IP:13389-1-AP, 4ug; Detection:13389-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse heart using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13389-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of BxPC-3 using 13389-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed BxPC-3 cells using PFKP antibody (13389-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13389-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PFKP antibody (13389-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules BxPC-3, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules Raji, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules Jurkat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu de cancer du poumon humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

13389-1-AP cible PFKP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PFKP Protéine recombinante Ag4047
• Nom complet: phosphofructokinase, platelet
• Masse moléculaire calculée: 784 aa, 86 kDa
• Poids moléculaire observé: 80-86 kDa
• Numéro d’acquisition GenBank: BC029138
• Symbole du gène: PFKP
• Identification du gène (NCBI): 5214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).

Publications

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
mouse	WB	Cell Metab; Augmentation of scleral glycolysis promotes myopia through histone lactylation; Authors - Xiaolei Lin
mouse	WB,IF	Nat Metab; Time of exercise differentially impacts bone growth in mice; Authors - Shaoling Yu
human	WB	Mol Cell; R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis.; Authors - Ying Qing ; KD Validated
human	WB	Stem Cell Reports; The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway.; Authors - Zihao Sun
mouse	WB	Acta Pharmacol Sin; Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis.; Authors - Lei Xu