Anticorps Polyclonal de lapin anti-PERK/EIF2AK3

• Phare
• Validé par KD/KO

PERK/EIF2AK3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

96

Avis (2)

N° de cat : 20582-1-AP

Synonymes

EIF2AK3, PERK, Pancreatic eIF2 alpha kinase, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3

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Galerie de données de validation

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  WB analysis using 20582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20582-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 20582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20582-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 20582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20582-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20582-1-AP

  Immunofluorescent analysis of HeLa cells using 20582-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 20582-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PERK/EIF2AK3 (20582-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 94 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

20582-1-AP cible PERK/EIF2AK3 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic translation initiation factor 2-alpha kinase 3
• Masse moléculaire calculée: 125 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: NM_004836
• Symbole du gène: PERK
• Identification du gène (NCBI): 9451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts potentially as a metabolic sensor in the INS-secreting beta-cells to modulate the trafficking and quality control of pro-INS in the ER relative to the physiological demands for circulating INS (PMID:20530744). PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170 kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327). This antibody is specific to PERK.

Publications

Species	Application	Title
	WB	Nat Commun; Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy.; Authors - Davy Vanhoutte
	WB	Oxid Med Cell Longev; Curcumin in Combination with Aerobic Exercise Improves Follicular Dysfunction via Inhibition of the Hyperandrogen-Induced IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in PCOS-Like Rats.; Authors - Yaling Zhang
human	WB,IHC	Phytother Res; Activation of endoplasmic reticulum stress by harmine suppresses the growth of esophageal squamous cell carcinoma; Authors - Ronghui Fan
human	WB	Cell Death Discov; Cysteine dioxygenase 1 attenuates the proliferation via inducing oxidative stress and integrated stress response in gastric cancer cells; Authors - Gang Ma ; KD Validated
human	WB	Mol Oncol; The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myeloma; Authors - George Luo ; KO Validated
human	WB	J Med Chem; Cytotoxic BODIPY-Appended Half-Sandwich Iridium(III) Complex Forms Protein Adducts and Induces ER Stress.; Authors - Robin Ramos

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Maria (Verified Customer) (02-10-2021)

PERK in human primary fibroblasts. 12% TGX gel. Blocking 1h RT 3% BSA in PBST (0.1% Tween). Primary incubation 16h 4ºC 1:1000. Secondary 1:5000 Goat anti-rabbit HRP 1h RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Primary Fibroblasts

PERK/EIF2AK3 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:20582-1-AP)

FH

Aeid (Verified Customer) (11-12-2018)

Works fine for HEK293 but not for MEF or INS1 cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, MEF, INS1