Anticorps Polyclonal de lapin anti-PDSS2

• Phare
• Validé par KD/KO

PDSS2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 13544-1-AP

Synonymes

C6orf210, DLP1, hDLP1, PDSS2

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Galerie de données de validation

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  WB analysis of A375 using 13544-1-AP

  WB result of PDSS2 antibody (13544-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDSS2 transfected A375 cells.

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  WB analysis of SGC-7901 using 13544-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13544-1-AP (PDSS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 13544-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13544-1-AP (PDSS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules SGC-7901, cellules A375
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du foie humain, tissu hépatique de souris, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

13544-1-AP cible PDSS2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDSS2 Protéine recombinante Ag4487
• Nom complet: prenyl (decaprenyl) diphosphate synthase, subunit 2
• Masse moléculaire calculée: 399 aa, 44 kDa
• Poids moléculaire observé: 27-30 kDa, 44-47 kDa
• Numéro d’acquisition GenBank: BC039906
• Symbole du gène: PDSS2
• Identification du gène (NCBI): 57107
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDSS2, also named as C6orf210, is required for the synthesis of coenzyme Q10 (CoQ10), which is synthesized in the mitochondrial inner membrane and plays a vital role in the mitochondrial respiratory chain, pyrimidine nucleoside biosynthesis, and modulation of apoptosis (PMID:25330808). An increasing body of evidence suggests that PDSS2 is a tumor suppressor gene in certain cancers, such as melanoma, gastric cancer and non-small cell lung cancer. Besides, PDSS2 could be used as a prognostic biomarker for HCC (PMID:25780306). PDSS2 has 2 isoforms with MW of 44 kDa and 27 kDa while 13544-1-AP antibody detects the bands around 27-30 kDa and 44-47 kDa in SDS-PAGE.

Publications

Species	Application	Title
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB, IF	Aging Cell; Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging.; Authors - Assaf Ben-Meir
human	WB	Neurol Genet; Novel Homozygous Variant in COQ7 in Siblings With Hereditary Motor Neuropathy; Authors - Ian C Smith
human	WB	J Assist Reprod Genet; PMID: 26552664; Authors - Yaakov Bentov
human	WB	Mol Genet Metab Rep; A novel COQ7 mutation causing primarily neuromuscular pathology and its treatment options; Authors - Ying Wang
mouse	IF	Antioxidants (Basel); Co-Enzyme Q10 Supplementation Rescues Cumulus Cells Dysfunction in a Maternal Aging Model.; Authors - Assaf Ben-Meir