Anticorps Polyclonal de lapin anti-PDRG1

PDRG1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16968-1-AP

Synonymes

C20orf126, p53 and DNA damage regulated 1, PDRG, PDRG1

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Galerie de données de validation

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  WB analysis of Jurkat using 16968-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16968-1-AP (PDRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16968-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16968-1-AP (PDRG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 16968-1-AP

  Immunofluorescent analysis of HepG2 cells, using PDRG1 antibody 16968-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

16968-1-AP cible PDRG1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDRG1 Protéine recombinante Ag9109
• Nom complet: p53 and DNA damage regulated 1
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC009334
• Symbole du gène: PDRG1
• Identification du gène (NCBI): 81572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDRG1 codes for a novel protein called p53 and DNA damage-regulated protein 1. Human PDRG1 is predominantly expressed in normal testis and its expression can be induced by UV irradiation and be repressed by p53 [PMID14562055]. Recently, PDRG1 was identified as a novel tumor marker for multiple malignancies that is selectively regulated by genotoxic stress. Its expression was upregulated in multiple malignancies including cancers of the colon, rectum, ovary, lung, stomach, breast and uterus when compared to normal tissues [PMID: 21193842], suggesting its being a novel tumor marker that could play a role in cancer development and/or progression.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.; Authors - Paul Yenerall
mouse	IHC	DNA Repair (Amst); The influence of retinoic acid-induced differentiation on the radiation response of male germline stem cells.; Authors - Yi Zheng
human	WB	Ann Transl Med; A pan-cancer analysis of the expression and prognostic significance of PDRG1; Authors - Zhen Liu
Artemia sinica	WB	Int J Mol Sci; The Potential Roles of the Apoptosis-Related Protein PDRG1 in Diapause Embryo Restarting of Artemia sinica.; Authors - Wan Zhang