Anticorps Polyclonal de lapin anti-PDK4

• Phare
• Validé par KD/KO

PDK4 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

62

Avis (1)

N° de cat : 12949-1-AP

Synonymes

PDK4

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Galerie de données de validation

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  WB analysis using 12949-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 12949-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12949-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12949-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 12949-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12949-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 12949-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12949-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12949-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 12949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12949-1-AP (PDK4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules NCI-H1299, tissu cardiaque de rat, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cardiaque de rat, tissu cardiaque de souris, tissu de cancer du pancréas humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 56 publications below
• IHC: See 7 publications below
• IF: See 8 publications below
• ELISA: See 1 publications below

Informations sur le produit

12949-1-AP cible PDK4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDK4 Protéine recombinante Ag3629
• Nom complet: pyruvate dehydrogenase kinase, isozyme 4
• Masse moléculaire calculée: 411 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC040239
• Symbole du gène: PDK4
• Identification du gène (NCBI): 5166
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.; Authors - Li Wang
human	WB	Sci Transl Med; Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice.; Authors - Biying Zhu
human	WB	Mol Syst Biol; STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.; Authors - Monika Oberhuber
human	WB	Theranostics; ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.; Authors - Xiaoxin Zhang
mouse	IF	Diabetes; Glucose controls glucagon secretion by regulating fatty acid oxidation in pancreatic alpha cells; Authors - Sarah L Armour
mouse	WB	PLoS Genet; PMID: 27711113; Authors - Xiaolin Lin ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (01-30-2020)

Strong, sharp band as predicted size around 50kDa. It downregulates in DCM samples.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart