Anticorps Polyclonal de lapin anti-PDIA6

• Phare
• Validé par KD/KO

PDIA6 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 18233-1-AP

Synonymes

ER protein 5, ERP5, P5, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase P5, TXNDC7

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Galerie de données de validation

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  WB analysis using 18233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18233-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18233-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18233-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18233-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18233-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 18233-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18233-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 18233-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18233-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18233-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18233-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18233-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 18233-1-AP

  IP result of anti-PDIA6 (IP:18233-1-AP, 4ug; Detection:18233-1-AP 1:3000) with HEK-293 cells lysate 3000ug.

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  IHC staining of human stomach using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 18233-1-AP

  Immunofluorescent analysis of HeLa cells using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 18233-1-AP

  Immunofluorescent analysis of HeLa cells using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de rat, cellules HEK-293, cellules HeLa, cellules L02, tissu cardiaque de souris, tissu hépatique de souris, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer de l'estomac humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

18233-1-AP cible PDIA6 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDIA6 Protéine recombinante Ag12940
• Nom complet: protein disulfide isomerase family A, member 6
• Masse moléculaire calculée: 48 kDa, 54 kDa
• Poids moléculaire observé: 48-54 kDa
• Numéro d’acquisition GenBank: BC001312
• Symbole du gène: PDIA6
• Identification du gène (NCBI): 10130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.

Publications

Species	Application	Title
human	IHC	Cancer Cell; p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.; Authors - Alessandro Carugo
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
mouse	WB	J Exp Med; Essential cell-extrinsic requirement for PDIA6 in lymphoid and myeloid development.; Authors - Jin Huk Choi
human	WB	Nucleic Acids Res; Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis.; Authors - Neus Mestre-Farràs ; KD Validated
human	WB	J Immunother Cancer; MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells; Authors - Yoshihiro Morimoto
human	WB,IF,IP	Antiviral Res; Venezuelan equine encephalitis virus E1 protein interacts with PDIA6 and PDI inhibition reduces alphavirus production; Authors - Lauren Panny