Anticorps Monoclonal anti-ERp57/ERp60

ERp57/ERp60 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

3G4G7

N° de cat : 66423-1-Ig

Synonymes

58 kDa microsomal protein, Cell and organelle markers, Disulfide isomerase ER 60, endoplasmic reticulum marker, ER protein 57, ER protein 60, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3

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Galerie de données de validation

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  WB analysis using 66423-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HeLa using 66423-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66423-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66423-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66423-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66423-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66423-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66423-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66423-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 66423-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66423-1-Ig cible ERp57/ERp60 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERp57/ERp60 Protéine recombinante Ag8741
• Nom complet: protein disulfide isomerase family A, member 3
• Masse moléculaire calculée: 505 aa, 57 kDa
• Poids moléculaire observé: 57 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC014433
• Symbole du gène: PDIA3
• Identification du gène (NCBI): 2923
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.