• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-PDH E1 Alpha

PDH E1 Alpha Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 18068-1-AP

Synonymes

PDH E1 alpha, PDH E1α, PDHA1, PDHE1 A type I, pyruvate dehydrogenase E1 α subunit



Applications testées

Résultats positifs en WBcellules HeLa, cellules HEK293, tissu cérébral de rat, tissu cérébral de souris, tissu rénal de rat
Résultats positifs en IPtissu rénal de souris
Résultats positifs en IHCtissu de cancer du foie humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

18068-1-AP cible PDH E1 Alpha dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, poulet, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PDH E1 Alpha Protéine recombinante Ag12701
Nom complet pyruvate dehydrogenase (lipoamide) alpha 1
Masse moléculaire calculée 43 kDa
Poids moléculaire observé 39-48 kDa
Numéro d’acquisition GenBankBC002406
Symbole du gène PDHA1
Identification du gène (NCBI) 5160
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing with the molecular mass of 39-48 kDa. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

Protocole

Product Specific Protocols
WB protocol for PDH E1 Alpha antibody 18068-1-APDownload protocol
IHC protocol for PDH E1 Alpha antibody 18068-1-APDownload protocol
IF protocol for PDH E1 Alpha antibody 18068-1-APDownload protocol
IP protocol for PDH E1 Alpha antibody 18068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
mouseWB

Nat Cell Biol

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

Authors -
  • KO Validated
ratWB

Cell Stem Cell

A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.

Authors - Jianyong Du
humanWB

Cell Metab

Environment Impacts the Metabolic Dependencies of Ras-Driven Non-Small Cell Lung Cancer.

Authors - Shawn M Davidson
  • KO Validated
humanWB

Mol Cell

Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.

Authors - Ji Zhang
  • KD Validated
humanWB

Circ Res

(Pro)renin Receptor Inhibition Reprograms Hepatic Lipid Metabolism and Protects Mice from Diet-Induced Obesity and Hepatosteatosis.

Authors - Liwei Ren

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

kes (Verified Customer) (01-17-2022)

I used it for WB(1:1000) and got nice bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Liver cells
PDH E1 Alpha Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:18068-1-AP)
FH

David (Verified Customer) (09-03-2019)

Good detection with 30µg of protein and dilution at 1/1000

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Brain
PDH E1 Alpha Antibody Western Blot, validation (1/1000 dilution) in Brain (Cat no:18068-1-AP)
FH

Svitlana (Verified Customer) (11-02-2017)

Loading:per well 15 ug/ulGradient gel 4-12%Transfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hImaging:Odyssey CLx Imaging

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Whole tissue homogenate (human, mouse), mitochondria (human, mouse), whole cell homogenate (rat cell line), mitochondria (rat cell line).
PDH E1 Alpha Antibody Western Blot validation (1:1000 dilution) in Whole tissue homogenate (human, mouse), mitochondria (human, mouse), whole cell homogenate (rat cell line), mitochondria (rat cell line). (Cat no:18068-1-AP)