Anticorps Polyclonal de lapin anti-PDCD6

• Phare
• Validé par KD/KO

PDCD6 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 12303-1-AP

Synonymes

ALG 2, ALG2, PDCD6, PEF1B, programmed cell death 6

---

Galerie de données de validation

• 

  WB analysis using 12303-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12303-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12303-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

• 

  IP experiment of HeLa using 12303-1-AP

  IP result of anti-PDCD6 (IP:12303-1-AP, 3ug; Detection:12303-1-AP 1:500) with HeLa cells lysate 3200ug.

• 

  IHC staining of human lung cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules NIH/3T3, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

12303-1-AP cible PDCD6 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDCD6 Protéine recombinante Ag2949
• Nom complet: programmed cell death 6
• Masse moléculaire calculée: 191 aa, 22 kDa
• Poids moléculaire observé: 15-22 kDa
• Numéro d’acquisition GenBank: BC012384
• Symbole du gène: PDCD6
• Identification du gène (NCBI): 10016
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nature; Structural basis for dimerization quality control.; Authors - Elijah L Mena
human	IF	Science; Triggered recruitment of ESCRT machinery promotes endolysosomal repair.; Authors - Michael L Skowyra
human	WB,IF	Cell; Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.; Authors - Colleen A McGourty ; KD Validated
human	WB	Mol Cell; Co-adaptor driven assembly of a CUL3 E3 ligase complex.; Authors - David Akopian
human	WB,IF	Cell Rep; Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.; Authors - Johannes Westman ; KD Validated
human	IF	J Cell Biol; Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters; Authors - Femmy C Stempels