Anticorps Polyclonal de lapin anti-PDCD4

• Phare
• Validé par KD/KO

PDCD4 Polyclonal Antibody for WB, IP, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA, FC (Intra)

Conjugaison

Non conjugué

Publications

32

N° de cat : 12587-1-AP

Synonymes

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a

---

Galerie de données de validation

• 

  WB analysis using 12587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK293 cells using 12587-1-AP

  WB result of PDCD4 antibody (12587-1-AP, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells.

• 

  WB analysis of MCF-7 using 12587-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12587-1-AP

  IP result of anti-PDCD4 (IP:12587-1-AP, 4ug; Detection:12587-1-AP 1:3000) with HeLa cells lysate 4000ug.

• 

  IHC staining of human breast cancer using 12587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12587-1-AP (PDCD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  FC experiment of MCF-7 using 12587-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PDCD4 (12587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HEK293, cellules HEK-293, cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 28 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Informations sur le produit

12587-1-AP cible PDCD4 dans les applications de WB, IP, IF, IHC, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDCD4 Protéine recombinante Ag3288
• Nom complet: programmed cell death 4 (neoplastic transformation inhibitor)
• Masse moléculaire calculée: 469 aa, 52 kDa
• Poids moléculaire observé: 54-64 kDa
• Numéro d’acquisition GenBank: BC026104
• Symbole du gène: PDCD4
• Identification du gène (NCBI): 27250
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.

Publications

Species	Application	Title
human	WB,IHC	J Nanobiotechnology; Cold exposure protects against medial arterial calcification development via autophagy; Authors - Fu-Xing-Zi Li
human	WB	Oncogene; miR-21a in exosomes from Lewis lung carcinoma cells accelerates tumor growth through targeting PDCD4 to enhance expansion of myeloid-derived suppressor cells.; Authors - Xingju Zhang ; KD Validated
mouse	WB,IHC	Clin Transl Med; Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.; Authors - Yunqiang He
rat	WB	Biomed Pharmacother; MiR-208a-3p aggravates autophagy through the PDCD4-ATG5 pathway in Ang II-induced H9c2 cardiomyoblasts.; Authors - Li Wang ; KD Validated
rat,mouse	WB,IHC,IF	Oxid Med Cell Longev; Protective Effects of PPARγ on Renal Ischemia-Reperfusion Injury by Regulating miR-21; Authors - Ruizhen Huang
		Cell Cycle; Resveratrol prevents rapamycin-induced upregulation of autophagy and selectively induces apoptosis in TSC2-deficient cells.; Authors - Anya Alayev