Anticorps Polyclonal de lapin anti-PD-L2/CD273

PD-L2/CD273 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 18251-1-AP

Synonymes

B7 DC, Btdc, Butyrophilin B7 DC, CD273, PD 1 ligand 2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, PDL2, PD-L2, PD-L2/CD273, Programmed death ligand 2

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Galerie de données de validation

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  WB analysis using 18251-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18251-1-AP (PD-L2/CD273 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 18251-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 18251-1-AP (PD-L2/CD273 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18251-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18251-1-AP (PD-L2/CD273 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18251-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18251-1-AP (PD-L2/CD273 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HepG2, cellules RAW 264.7, tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 12 publications below

Informations sur le produit

18251-1-AP cible PD-L2/CD273 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PD-L2/CD273 Protéine recombinante Ag13039
• Nom complet: programmed cell death 1 ligand 2
• Masse moléculaire calculée: 273 aa, 31 kDa
• Poids moléculaire observé: 40-50 kDa, 28 kDa
• Numéro d’acquisition GenBank: BC074766
• Symbole du gène: PD-L2
• Identification du gène (NCBI): 80380
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Programmed cell death ligand 2 (PD-L2, also known as CD273, or B7-DC), is one of the B7 family molecules which are type I transmembrane proteins belonging to the immunoglobulin superfamily. PD-L2 was identified both by its homology to PD-L1 and by analysis of genes differentially expressed in libraries generated from dendritic cells (DC) and activated macrophages (PMID: 14515254). PD-L2 is a second ligand for PD-1, a costimulatory molecule that plays an inhibitory role in regulating T cell activation in the periphery.

Publications

Species	Application	Title
human	WB,IHC	Aging Cell; Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression.; Authors - Qixia Xu
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	IHC	Cancer Sci; ANO9 regulates PD-L2 expression and binding ability to PD-1 in gastric cancer.; Authors - Keita Katsurahara
human	IHC	J Cell Mol Med; IFI30 expression is an independent unfavourable prognostic factor in glioma.; Authors - Xiu Liu
human	WB	J Transl Med; Treatment with eFT-508 increases chemosensitivity in breast cancer cells by modulating the tumor microenvironment.; Authors - Zhao-Ying Yang
human	IHC	Oncol Rep; Differential expression of PD‑L1 and PD‑L2 is associated with the tumor microenvironment of TILs and M2 TAMs and tumor differentiation in non‑small cell lung cancer.; Authors - Ryota Sumitomo