Anticorps Polyclonal de lapin anti-PD-ECGF

PD-ECGF Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12383-1-AP

Synonymes

ECGF1, Gliostatin, hPD ECGF, MNGIE, PD ECGF, PDECGF, PD-ECGF, TdRPase, thymidine phosphorylase, TP, TYMP

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Galerie de données de validation

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  WB analysis using 12383-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12383-1-AP (PD-ECGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12383-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12383-1-AP (PD-ECGF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12383-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12383-1-AP (PD-ECGF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 12383-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12383-1-AP (PD-ECGF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12383-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12383-1-AP (PD-ECGF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12383-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12383-1-AP (PD-ECGF antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de l'estomac humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

12383-1-AP cible PD-ECGF dans les applications de WB, IF, FC, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PD-ECGF Protéine recombinante Ag3052
• Nom complet: thymidine phosphorylase
• Masse moléculaire calculée: 482 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC018160
• Symbole du gène: Thymidine phosphorylase
• Identification du gène (NCBI): 1890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Platelet derived endothelial cell growth factor (PD-ECGF), also known as thymidine phosphorylase (TP), induces migration and angiogenesis in endothelial and tumor cells, and was upregulated in various malignancies compared to that in normal tissues. Interestingly, PD-ECGF has dual effect on tumor development and chemotherapy. It could stimulate cancer cell migration and proliferation. On the other hand, some chemotherapeutic agents (5-fluorouracil, capecitabine, etc.) were converted to their active forms through TP enzymes.

Publications

Species	Application	Title
human	WB	Oncogene; The proteomic characterization of the peritumor microenvironment in human hepatocellular carcinoma.; Authors - Yuhan Gu
human	FC	Oncotarget; PMID: 29707135; Authors - Toshiki Iwai
mouse	WB,IF	Acta Pharmacol Sin; Exploring the protective effects of schizandrol A in acute myocardial ischemia mice by comprehensive metabolomics profiling integrated with molecular mechanism studies.; Authors - Qiong Lai
mouse	WB	J Ovarian Res; 4-Methylumbelliferone inhibits ovarian cancer growth by suppressing thymidine phosphorylase expression.; Authors - Ryosuke Tamura
human	IHC	Mol Imaging Biol; Evaluation of [(18)F]Fluorothymidine as a Biomarker for Early Therapy Response in a Mouse Model of Colorectal Cancer.; Authors - Sara Rapic
mouse	WB	Antioxidants (Basel); D-Penicillamine Reveals the Amelioration of Seizure-Induced Neuronal Injury via Inhibiting Aqp11-Dependent Ferroptosis; Authors - Nan Yang