Anticorps Polyclonal de lapin anti-PCSK5

• Phare
• Validé par KD/KO

PCSK5 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 16470-1-AP

Synonymes

hPC6, PC5, PC6, PC6A, PCSK5, Proprotein convertase 5, Proprotein convertase 6, SPC6

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Galerie de données de validation

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  IHC staining of human kidney using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16470-1-AP (PCSK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16470-1-AP (PCSK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16470-1-AP (PCSK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16470-1-AP (PCSK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16470-1-AP (PC6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16470-1-AP (PC6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16470-1-AP (PC6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16470-1-AP (PC6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human spleen using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu cérébral de souris, tissu cérébral humain, tissu cutané humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

16470-1-AP cible PCSK5 dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCSK5 Protéine recombinante Ag9552
• Nom complet: proprotein convertase subtilisin/kexin type 5
• Masse moléculaire calculée: 102 kDa, 207 kDa
• Poids moléculaire observé: 210 kDa
• Numéro d’acquisition GenBank: BC012064
• Symbole du gène: PCSK5
• Identification du gène (NCBI): 5125
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PC5 ,a mammalian kex2-like processing endoprotease, mediates posttranslational endoproteolytic processing for several integrin alpha subunits.It has been identified in rat and mouse endocrine and neuroendocrine tissues,and is encoded by multiple mRNAs that generate two splice variant isoforms referred to as PC5A (102KDa) and PC5B (207KDa) .PC5A is smaller than PC5B and it can be processed into two forms (117 kDa and 65 kDa) in neuronal cells(PMID:11457520).

Publications

Species	Application	Title
rat		Mol Med Rep; MicroRNA‑338‑3p regulates age‑associated osteoporosis via targeting PCSK5.; Authors - Jie Tong ; KD Validated
human		Biochem Biophys Rep; Secreted factors from adipose tissue-derived mesenchymal stem cells suppress oxygen/glucose deprivation-induced cardiomyocyte cell death via furin/PCSK-like enzyme activity.; Authors - Yuki Tachida
human	IHC	Mol Cell Biochem; PCSK5 downregulation promotes the inhibitory effect of andrographolide on glioblastoma through regulating STAT3; Authors - Huiyuan Gong