Anticorps Polyclonal de lapin anti-PCP4

PCP4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 14705-1-AP

Synonymes

Brain specific antigen PCP 4, PCP4, PEP 19, PEP19, Purkinje cell protein 4

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Galerie de données de validation

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  WB analysis using 14705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14705-1-AP (PCP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14705-1-AP

  IP result of anti-PCP4 (IP:14705-1-AP, 4ug; Detection:14705-1-AP 1:1000) with mouse brain tissue lysate 4000 ug.

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  IHC staining of mouse cerebellum using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14705-1-AP (PCP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14705-1-AP (PCP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14705-1-AP (PCP4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14705-1-AP (PCP4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14705-1-AP (PCP4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14705-1-AP (PCP4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 14705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PCP4 antibody (14705-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 14705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PCP4 antibody (14705-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cerveau de rat
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cervelet humain, tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

14705-1-AP cible PCP4 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCP4 Protéine recombinante Ag6388
• Nom complet: Purkinje cell protein 4
• Masse moléculaire calculée: 7 kDa
• Poids moléculaire observé: 8 kDa
• Numéro d’acquisition GenBank: BC013791
• Symbole du gène: PCP4
• Identification du gène (NCBI): 5121
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PCP4, also named as PEP19, belongs to a family of proteins involved in calcium transduction signals and binds calmodulin via an IQ motif, in a calcium independent manner. It is mainly expressed in ectoderm and neuroectoderm comprising neural crest derived cells.

Publications

Species	Application	Title
human	IF	Cancer Cell; Single-Cell Characterization of Malignant Phenotypes and Developmental Trajectories of Adrenal Neuroblastoma.; Authors - Rui Dong
human	IF	Cell Stem Cell; Human brain organoids assemble functionally integrated bilateral optic vesicles.; Authors - Elke Gabriel
human	WB	Ann Neurol; PMID: 32167609; Authors - Jimena Monzón-Sandoval
rat	IF	Nat Neurosci; Acute silencing of hippocampal CA3 reveals a dominant role in place field responses.; Authors - Heydar Davoudi
human	IHC	Biomedicines; Characteristic Analysis of Featured Genes Associated with Cholangiocarcinoma Progression; Authors - Qigu Yao
human	IF	Nat Protoc; Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia; Authors - Elke Gabriel