Anticorps Polyclonal de lapin anti-PCIF1

PCIF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 16082-1-AP

Synonymes

C20orf67, CAPAM, Cap-specific adenosine methyltransferase, EC:2.1.1.62, hCAPAM

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Galerie de données de validation

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  WB analysis using 16082-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 16082-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16082-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16082-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 16082-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 16082-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 16082-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCIF1 antibody (16082-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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  IF Staining of Caco-2 using 16082-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PCIF1 antibody (16082-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Ramos, cellules HEK-293T, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules Caco-2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

16082-1-AP cible PCIF1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCIF1 Protéine recombinante Ag9080
• Nom complet: PDX1 C-terminal inhibiting factor 1
• Masse moléculaire calculée: 704 aa, 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC013365
• Symbole du gène: PCIF1
• Identification du gène (NCBI): 63935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse,human	WB,IP	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
human	WB	Nat Commun; HIV reprograms host m 6 Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1; Authors - Qiong Zhang
	WB	J Virol; The pseudorabies virus UL13 protein kinase triggers phosphorylation of the RNA demethylase FTO, which is associated with FTO-dependent suppression of interferon-stimulated gene expression; Authors - Ruth Verhamme
pig	WB	Cell Rep; Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex.; Authors - Robert J J Jansens
mouse,human	WB,IF	EMBO J; Role of PCIF1-mediated 5\'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy; Authors - Lingling Wang
human	WB	Proc Natl Acad Sci U S A; PCIF1-mediated deposition of 5'-cap N6,2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection; Authors - Lingling Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anirban (Verified Customer) (01-25-2023)

blocking 1 hr in 5% milk/TBST. Primary ab- 1/1000 in 3% BSA/TBST at 4 deg for 16 hrs, Secondary ab-1/5000 in 5% milk/TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: 786-O, 769p

PCIF1 Antibody Western Blot validation (1/1000 dilution) in 786-O, 769p (Cat no:16082-1-AP)

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