Anticorps Polyclonal de lapin anti-PCGF6

• Phare
• Validé par KD/KO

PCGF6 Polyclonal Antibody for WB, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, CoIP, ChIP, ELISA, FC (Intra)

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 24103-1-AP

Synonymes

RNF134, PCGF 6, Mel18 and Bmi1-like RING finger, MBLR

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Galerie de données de validation

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  WB analysis of HEK-293 using 24103-1-AP

  WB result of PCGF6 antibody (24103-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCGF6 transfected HEK-293 cells.

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  WB analysis using 24103-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24103-1-AP (PCGF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 24103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24103-1-AP (PCGF6 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24103-1-AP (PCGF6 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HEK-293T using 24103-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human PCGF6 (24103-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK-293T, cellules PC-13, tissu hépatique de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HEK-293T

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4800
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

24103-1-AP cible PCGF6 dans les applications de WB, IP, IHC, CoIP, ChIP, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCGF6 Protéine recombinante Ag21124
• Nom complet: polycomb group ring finger 6
• Masse moléculaire calculée: 352 aa, 39 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC010235
• Symbole du gène: PCGF6
• Identification du gène (NCBI): 84108
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PCGF6, also named as MBLR or RNF134, is a 350 amino acid protein, which contains one RING-type zinc finger. PCGF6 localizes in the nucleus and is widely expressed in many tissues. PCGF6 as a transcriptional repressor may modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase.

Publications

Species	Application	Title
		Cell Stem Cell; SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States.; Authors - Jack-Christophe Cossec
mouse		Sci Adv; The SAM domain-containing protein 1 (SAMD1) acts as a repressive chromatin regulator at unmethylated CpG islands.; Authors - Bastian Stielow
mouse	WB	Nat Commun; E2F6 initiates stable epigenetic silencing of germline genes during embryonic development.; Authors - Thomas Dahlet
mouse	WB	Nat Commun; Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing.; Authors - Kentaro Mochizuki ; KO Validated
human	WB,CoIP	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan ; KD Validated
human	WB	Elife; Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.; Authors - Haritha Mathsyaraja

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Aktan (Verified Customer) (12-24-2019)

The blot is subcellular fractionation of the cell nuclei using increasing concentrations of salt. Last two lanes are chromatin fractions. After electrophoresis and transfer, 5%BSA in PBST was used as blocker for 1h. The primary antibody is diluted in blocker 1:500 and the blot is incubated o/n at cold. The bands are detected using licor secondary antibodies and Licor imager.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: E14Tg mouse embryonic stem cells

PCGF6 Antibody Western Blot, validation (1:500 dilution) in E14Tg mouse embryonic stem cells (Cat no:24103-1-AP)