Anticorps Polyclonal de lapin anti-PCCA

PCCA Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 21988-1-AP

Synonymes

PCCA, PCCase subunit alpha

---

Galerie de données de validation

• 

  WB analysis using 21988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21988-1-AP (PCCA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21988-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21988-1-AP (PCCA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21988-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21988-1-AP (PCCA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21988-1-AP (PCCA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 21988-1-AP

  IP result of anti-PCCA (IP:21988-1-AP, 4ug; Detection:21988-1-AP 1:1000) with L02 cells lysate 1800ug.

• 

  IHC staining of human liver cancer using 21988-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21988-1-AP (PCCA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21988-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21988-1-AP (PCCA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21988-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCCA antibody (21988-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules L02, cellules NIH/3T3, tissu de muscle squelettique de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 2 publications below

Informations sur le produit

21988-1-AP cible PCCA dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCCA Protéine recombinante Ag17271
• Nom complet: propionyl Coenzyme A carboxylase, alpha polypeptide
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC000140
• Symbole du gène: PCCA
• Identification du gène (NCBI): 5095
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Propionyl-CoA carboxylase (PCC) catalyzes the biotin-dependent carboxylation of propionyl-CoA to D-methyl-malonyl CoA, a reaction that occurs in the mitochondrial matrix. PCC is involved in the catabolism of several essential amino acids (methionine, isoleucine, threonine and valine), as well as odd chain fatty acids and cholesterol. Deficiency of PCC results in propionic acidemia, a metabolic disorder characterized by severe metabolic ketoacidosis, vomiting, lethargy and hypotonia. PCC consists of nonidentical subunits (α and β) encoded by different genes (PCCA and PCCB, respectively). The αPCC cDNA contains an open reading frame of 2106 nucleotide bases and codes for a 702 amino acid polypeptide. The mature length subunit is 70 kDa and contains the biotin binding site.(PMID:8852656). This protein has 3 isoforms produced by alternative splicing with the molecular weight of 80 kDa, 77 kDa and 75 kDa. The full length protein has a transit peptide with 52 amino acids which will be removed in mature form. This antibody is specific to PCCA.

Publications

Species	Application	Title
human	IF	Nat Commun; Dual mRNA therapy restores metabolic function in long-term studies in mice with propionic acidemia.; Authors - Lei Jiang
mouse	WB	J Cell Biol; CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.; Authors - Désirée Schatton
mouse	WB	Mol Genet Metab; Metabolic perturbations mediated by propionyl-CoA accumulation in organs of mouse model of propionic acidemia.; Authors - Wentao He
mouse	WB	Hum Gene Ther; Effects of Adeno-associated Virus Serotype and Tissue-specific Expression on Circulating Biomarkers of Propionic Acidemia.; Authors - Adam Guenzel
mouse	WB	Hum Gene Ther; Long-term Sex-Biased Correction of Circulating Propionic Acidemia Disease Markers by Adeno-Associated Virus Vectors.; Authors - Adam Guenzel
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali