Anticorps Polyclonal de lapin anti-PBX4

PBX4 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 22323-1-AP

Synonymes

Homeobox protein PBX4, PBX4, pre B cell leukemia homeobox 4

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Galerie de données de validation

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  WB analysis of HEK-293 using 22323-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 22323-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22323-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 22323-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 22323-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A2780 using 22323-1-AP

  IP result of anti-PBX4 (IP:22323-1-AP, 4ug; Detection:22323-1-AP 1:300) with A2780 cells lysate 3600 ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A2780, cellules COLO 320, cellules Jurkat, cellules SKOV-3
• Résultats positifs en IP: cellules A2780,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22323-1-AP cible PBX4 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PBX4 Protéine recombinante Ag17768
• Nom complet: pre-B-cell leukemia homeobox 4
• Masse moléculaire calculée: 374 aa, 41 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC141859
• Symbole du gène: PBX4
• Identification du gène (NCBI): 80714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pre-B-cell leukemia homeobox 4 (PBX4), also named as Homeobox protein PBX4, is one of the pbx gene encode homeodomain containing transcriptional regulators that interact with other proteins to control embryogenesis and tumorigenesis. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase. PBX4 may form heterodimers with other homeobox genes (hoxb1b or meis3) to specify different developmental fates during vertebrate embryogenesis (PMID:10679934). The trimeric complex containing Pbx4, Meis3, and Hoxb1b was also formed. The calculated molecular weight of PBX4 is about 41 kDa, but the molecular weight of heterodimers (PBX4 and hoxb1b) is about 70 kDa.