Anticorps Polyclonal de lapin anti-PAX7

PAX7 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (1)

N° de cat : 20570-1-AP

Synonymes

HUP1, paired box 7, Paired box protein Pax 7, PAX7, PAX7B

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Galerie de données de validation

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  WB analysis of NCCIT using 20570-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 20570-1-AP (PAX7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellule NCCIT, cellules NCCIT

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 11 publications below

Informations sur le produit

20570-1-AP cible PAX7 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain, porc, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: paired box 7
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: NM_002584
• Symbole du gène: PAX7
• Identification du gène (NCBI): 5081
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pax7 is a highly conserved transcription factor involved in early development of the neural crest and gastrulation and is a commonly used marker for satellite cells in muscle tissue. This antibody is specific to PAX7. It will not cross-react with other PAX protein family members.

What is the molecular weight of Pax7?

57 kDa

What is the role of Pax7?

One of the Paired Box (Pax) transcription factors, which are key in early development and organogenesis in vertebrates, Pax7 binds directly to DNA and is key in neural crest induction (PMID: 26410165). One of the earlier markers in this phase of embryogenesis, Pax7 therefore influences the migration and proliferation of multiple lineages of cells (PMID: 22848431).

In adult tissue, Pax7 is expressed by the quiescent resident muscle stem cells, also known as satellite cells, making it a useful marker for this cell type. It is essential for their formation and maintenance in their niche. When activated, these cells can proliferate in the normal maintenance of muscle tissue or respond to injury in repair. The co-expression of Pax7 with MyoD in these activated satellite cells regulates muscle cell proliferation (PMID: 16608873).

What proteins does Pax7 interact with?

An orthologue of Pax7, another transcription factor of the same family, is Pax3. These two proteins can form a heterodimer, binding to DNA to work together in myogenesis but can also function independently and have overlapping roles (PMID: 16380438).

What is the role of Pax7 in disease?

Pax7 and Pax 3 have both been identified in tumor tissue from the childhood cancer alveolar rhabdomyosarcoma (ARMS), which affects skeletal muscle (PMID: 23206814).

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
	WB	J Cachexia Sarcopenia Muscle; circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.; Authors - Junyu Yan
	IF, WB	Theranostics; Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development.; Authors - Shuyu Guo
	WB	Front Cell Dev Biol; PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.; Authors - Ye-Ya Tan
	IF	iScience; KLF2 in Myeloid Lineage Cells Regulates the Innate Immune Response during Skeletal Muscle Injury and Regeneration.; Authors - Palanikumar Manoharan
	WB	Cells; Role of lncRNA Has2os in Skeletal Muscle Differentiation and Regeneration; Authors - Wanxin Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sahya (Verified Customer) (08-13-2018)

Protocol - Primary Antibody - 1 hourPBS washSecondary Antibody - 1 hour

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100 and 1:200
• Cell Tissue Type: Skeletal Muscle Satellite Cells