Anticorps Polyclonal de lapin anti-PAX3

PAX3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 21386-1-AP

Synonymes

CDHS, HUP2, paired box 3, Paired box protein Pax 3, PAX3, WS1

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Galerie de données de validation

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  WB analysis of mouse skin using 21386-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21386-1-AP (PAX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21386-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21386-1-AP (PAX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skin using 21386-1-AP

  IP result of anti-PAX3 (IP:21386-1-AP, 4ug; Detection:21386-1-AP 1:500) with mouse skin tissue lysate 3200ug.

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Applications testées

• Résultats positifs en WB: tissu cutané de souris, cellules HEK-293
• Résultats positifs en IP: tissu cutané de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

21386-1-AP cible PAX3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAX3 Protéine recombinante Ag16021
• Nom complet: paired box 3
• Masse moléculaire calculée: 484 aa, 53 kDa
• Poids moléculaire observé: 56-60 kDa
• Numéro d’acquisition GenBank: BC101301
• Symbole du gène: PAX3
• Identification du gène (NCBI): 5077
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.

Publications

Species	Application	Title
hamster	IHC	Birth Defects Res; Cigarette smoke induced neural tube defects by down-regulating noggin expression.; Authors - Chuanhui Yin