Anticorps Polyclonal de lapin anti-CPB1

CPB1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12600-1-AP

Synonymes

Carboxypeptidase B, carboxypeptidase B1 (tissue), CPB, CPB1, Pancreas specific protein, PASP, PCPB

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Galerie de données de validation

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  WB analysis of mouse pancreas using 12600-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12600-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 12600-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12600-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50.

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50.

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, tissu pancréatique de rat
• Résultats positifs en IHC: tissu pancréatique humain, tissu de cancer du pancréas humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

12600-1-AP cible CPB1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPB1 Protéine recombinante Ag3243
• Nom complet: carboxypeptidase B1 (tissue)
• Masse moléculaire calculée: 417 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC015338
• Symbole du gène: CPB1
• Identification du gène (NCBI): 1360
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PASP(pancreas-specific protein) is also named as CPB1, CPB, PCPB and belongs to the peptidase M14 family. This protein modulates the plasminogen system both in the matrix and on the cell surface, thus leading to the inhibition of endothelial cell movement and tube formation. It is initially described as a pancreatic metallocarboxypeptidase and is a marker for acute pancreatitis(PMID:19717511). The deduced 416-amino acid preproenzyme has a 15-amino acid signal peptide and a 95-amino acid activation peptide.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); Is Carboxypeptidase B1 a Prognostic Marker for Ductal Carcinoma In Situ?; Authors - Charu Kothari
human	WB,IHC	Transl Oncol; Label-Free Quantitative Proteomics Unravels Carboxypeptidases as the Novel Biomarker in Pancreatic Ductal Adenocarcinoma.; Authors - Yang Song
rat	WB	Arch Gynecol Obstet; Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling.; Authors - Xiaohua Li