Anticorps Polyclonal de lapin anti-PARP9

• Phare
• Validé par KD/KO

PARP9 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 17535-1-AP

Synonymes

B aggressive lymphoma protein, BAL, BAL1, PARP 9, PARP9, Poly [ADP ribose] polymerase 9

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Galerie de données de validation

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  WB analysis using 17535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17535-1-AP (PARP9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 17535-1-AP

  IP result of anti-PARP9 (IP:17535-1-AP, 4ug; Detection:17535-1-AP 1:500) with MCF-7 cells lysate 4000ug.

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  IHC staining of human spleen using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 17535-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules THP-1
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu splénique humain, tissu cardiaque humain, tissu cutané humain, tissu de lymphome humain, tissu ovarien humain, tissu pulmonaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

17535-1-AP cible PARP9 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PARP9 Protéine recombinante Ag11587
• Nom complet: poly (ADP-ribose) polymerase family, member 9
• Masse moléculaire calculée: 819 aa, 92 kDa
• Poids moléculaire observé: 88 kDa
• Numéro d’acquisition GenBank: BC039580
• Symbole du gène: PARP9
• Identification du gène (NCBI): 83666
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated by one of the 17 members of the poly(ADP-ribose) polymerases (PARP). PARP-9 belongs to the subfamily of macroPARPs, associating one to three macro domains to the PARP domain. Overexpression of PARP-9 stimulates cell migration in vitro, suggesting a role for PARP-9 in the promotion of malignant B cell migration and dissemination in high risk DLBCL. PARP-9 is also likely a transcription coactivator, its overexpression in B lymphocytes, stimulated by IFNγ, inducing the transcription of IFNγ-controlled genes.

Publications

Species	Application	Title
human	WB,IF	Mol Cell; Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9.; Authors - Chun-Song Yang
human	WB	Mol Ther; PMID: 29456019; Authors - Xinyi Wang
treeshrew	IF,WB	J Immunol; An Alternative Splicing of Tupaia STING Modulated Anti-RNA Virus Responses by Targeting MDA5-LGP2 and IRF3.; Authors - Ling Xu ; KD Validated
human	WB	Oncol Lett; PARP9 is overexpressed in human breast cancer and promotes cancer cell migration.; Authors - Xinghong Tang ; KD Validated
human	WB	J Mol Biol; KH-like domains in PARP9/DTX3L and PARP14 coordinate protein-protein interactions to promote cancer cell survival; Authors - Hadil Saleh ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hadil (Verified Customer) (05-25-2023)

I have had trouble detecting PARP9 signals with other PARP9 antibodies, however, this antibody is a game changer. It works well both at room temperature (1.5 -2 hrs) and also overnight at 1:500 dilution. Would highly recommend to anyone who's studying PARP9.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mammalian cell lines (including HeLa, HNSCC cells, HEK)