Anticorps Polyclonal de lapin anti-PARP2

PARP2 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 20555-1-AP

Synonymes

ADPRT2, ADPRT-2, ADPRTL2, ARTD2, hPARP-2, pADPRT-2, PARP2, PARP-2, Poly [ADP-ribose] polymerase 2

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Galerie de données de validation

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  WB analysis using 20555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20555-1-AP (PARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20555-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20555-1-AP (PARP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat brain using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20555-1-AP cible PARP2 dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: poly (ADP-ribose) polymerase 2
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 60-66 kDa
• Numéro d’acquisition GenBank: NM_005484
• Symbole du gène: PARP2
• Identification du gène (NCBI): 10038
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PRP2 catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor. The antibody is specific to PARP2 (C-terminal). PARP2 can be detected as about 62 kDa (PMID: 10364231).