Anticorps Polyclonal de lapin anti-PARP11

PARP11 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 16692-1-AP

Synonymes

C12orf6, PARP 11, PARP11

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Galerie de données de validation

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  WB analysis of PC-3 using 16692-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16692-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 16692-1-AP (PARP11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 16692-1-AP (PARP11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3, tissu cardiaque de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cutané humain, tissu de côlon humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16692-1-AP cible PARP11 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PARP11 Protéine recombinante Ag10053
• Nom complet: poly (ADP-ribose) polymerase family, member 11
• Masse moléculaire calculée: 331 aa, 39 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC017569
• Symbole du gène: PARP11
• Identification du gène (NCBI): 57097
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARP11, also named as ARTD11, belongs to the  ARTD/PARP family. PARP11 is a mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. PARP11 plays a role in nuclear envelope stability and nuclear remodeling during spermiogenesis (PMID: 25043379). PARP11 has 4 isoforms with the molecular mass of 28-40 kDa.