Anticorps Polyclonal de lapin anti-PALMD

• Phare
• Validé par KD/KO

PALMD Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16531-1-AP

Synonymes

C1orf11, PALMD, palmdelphin, PALML, Paralemnin like protein

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Galerie de données de validation

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  WB analysis of HeLa using 16531-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16531-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16531-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16531-1-AP

  IP result of anti-PALMD (IP:16531-1-AP, 5ug; Detection:16531-1-AP 1:500) with mouse heart tissue lysate 6000ug.

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  IP experiment using 16531-1-AP

  IP result of PALMD antibody (16531-1-AP for Ip and Detection) with mouse heart tissue lysate.

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  IHC staining of human liver using 16531-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16531-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cardiaque de souris,
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

16531-1-AP cible PALMD dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PALMD Protéine recombinante Ag9796
• Nom complet: palmdelphin
• Masse moléculaire calculée: 551 aa, 63 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC012570
• Symbole du gène: PALMD
• Identification du gène (NCBI): 54873
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Palmdelphin, encoded by PALMD gene, is a newly identified cytosolic isoform of paralemmin-1, a lipid raft-associated protein implicated in cell shape control. Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Previous finding suggest that palmdelphin may peripherally associate with endomembranes or cytoskeleton-linked structures. Palmdelphin appears to be 80 kDa in Western blotting, larger than the predicated of 63 kDa, and this may be due to the acidic nature of this protein or its posttranslational modification.

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.; Authors - Sean-Patrick Riechers
human	WB	Cell Death Dis; Palmdelphin, a novel target of p53 with Ser46 phosphorylation, controls cell death in response to DNA damage.; Authors - N Dashzeveg ; KD Validated
rat	WB,IF	Front Cell Neurosci; Role of a Pdlim5:PalmD complex in directing dendrite morphology; Authors - Yogesh Srivastava
human	WB	Front Oncol; Low Level of PALMD Contributes to the Metastasis of Uveal Melanoma.; Authors - Min-Yun Cai ; KD Validated
human	WB,IF	bioRxiv; Role of a Pdlim5:PalmD complex in directing dendrite morphology; Authors - Yogesh Srivastava
mouse	IF,IHC	Cell Rep; Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease; Authors - Min Yu