Anticorps Polyclonal de lapin anti-PAK2

PAK2 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 19979-1-AP

Synonymes

C t PAK2, Gamma PAK, p21 activated kinase 2, p27 PAK 2p34, p34, p58, PAK 2, PAK2, PAK65, PAKgamma, S6/H4 kinase

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Galerie de données de validation

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  WB analysis using 19979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19979-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 19979-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 19979-1-AP

  IP result of anti-PAK2 (IP:19979-1-AP, 4ug; Detection:19979-1-AP 1:2000) with K-562 cells lysate 2400 ug.

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  IF Staining of HeLa using 19979-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK2 antibody (19979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules PC-3, cellules Raji, tissu de muscle squelettique humain
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19979-1-AP cible PAK2 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: p21 protein (Cdc42/Rac)-activated kinase 2
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: NM_002577
• Symbole du gène: PAK2
• Identification du gène (NCBI): 5062
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAK2, also named as PAK65, PAKgamma, p58, PAK-2p27, PAK-2p24 and C-t-PAK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. PAK2 has several isoforms with the MW of 54-62 kDa and 41 kDa. The 62 kDa PAK2 is cleaved into a 34 kDa C terminal fragment and a 28 kDa N terminal fragment with a time course that parallels apoptotic death in Jurkat cells. (PMID:10200518). Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form. The antibody has no cross reaction to PAK1 and PAK3.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Frédéric (Verified Customer) (02-18-2022)

Je suis satisfait du résultat car j’ai pu détecter par Western-blot une seule bande de forte intensité d’environ 69 kDa sur un lysat de plaquette humaine (voir figure ci-jointe).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: lysat de plaquette humaine

PAK2 Antibody Western Blot validation (1/1000 dilution) in lysat de plaquette humaine (Cat no:19979-1-AP)