Anticorps Polyclonal de lapin anti-PAIP2

PAIP2 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15583-1-AP

Synonymes

PABP interacting protein 2, PAIP 2, PAIP2, PAIP2A

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Galerie de données de validation

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  WB analysis of A431 using 15583-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15583-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15583-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15583-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15583-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 15583-1-AP

  IP result of anti-PAIP2 (IP:15583-1-AP, 4ug; Detection:15583-1-AP 1:500) with A431 cells lysate 1200ug.

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  IF Staining of HeLa using 15583-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15583-1-AP (PAIP2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules Jurkat, tissu pulmonaire de souris
• Résultats positifs en IP: cellules A431
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

15583-1-AP cible PAIP2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAIP2 Protéine recombinante Ag7119
• Nom complet: poly(A) binding protein interacting protein 2
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC001716
• Symbole du gène: PAIP2
• Identification du gène (NCBI): 51247
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IHC	J Neurosci; Proteasome modulates positive and negative translational regulators in long-term synaptic plasticity.; Authors - Chenghai Dong
human	WB	Front Genet; PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration; Authors - Chenyu Wang