Anticorps Polyclonal de lapin anti-PAH

PAH Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 16347-1-AP

Synonymes

PAH, PH, Phe 4 monooxygenase, Phenylalanine 4 hydroxylase, phenylalanine hydroxylase, PKU, PKU1

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Galerie de données de validation

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  WB analysis of HepG2 using 16347-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16347-1-AP (PAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16347-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16347-1-AP (PAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16347-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16347-1-AP (PAH Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 16347-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16347-1-AP (PAH Antibody) at dilution of 1:200 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

16347-1-AP cible PAH dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAH Protéine recombinante Ag9541
• Nom complet: phenylalanine hydroxylase
• Masse moléculaire calculée: 452 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC026251
• Symbole du gène: PAH
• Identification du gène (NCBI): 5053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAH, also known as PH, belongs to the aromatic amino acid hydroxylase (AAAH) enzyme family. PAH catalyzes the conversion of L-phenylalanine (L-Phe) to L-tyrosine (L-Tyr) by para-hydroxylation of the aromatic side chain. PAH is primarily present in the liver, where removal of excess L-Phe prevents the neurotoxic effect of hyperphenylalaninemia (HPA). The calculated molecular weight of PAH is 52 kDa (PMID: 23457044).

Publications

Species	Application	Title
mouse	WB	Pharmacol Res; Medioresinol as a novel PGC-1α activator prevents pyroptosis of endothelial cells in ischemic stroke through PPARα-GOT1 axis.; Authors - Yunjie Wang
mouse	WB	Life Sci Alliance; A phosphoproteomic approach reveals that PKD3 controls PKA-mediated glucose and tyrosine metabolism.; Authors - Angel Loza-Valdes
human	WB	Adv Sci (Weinh); A Metabolic Reprogramming Amino Acid Polymer as an Immunosurveillance Activator and Leukemia Targeting Drug Carrier for T‐Cell Acute Lymphoblastic Leukemia; Authors - Changzheng Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Angel (Verified Customer) (11-27-2020)

This antibody detects PAH in liver tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver tissue

PAH Antibody Western Blot validation (1:1000 dilution) in Liver tissue (Cat no:16347-1-AP)