Anticorps Polyclonal de lapin anti-PAF1

• Phare
• Validé par KD/KO

PAF1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 15441-1-AP

Synonymes

F23149_1, hPAF1, PAF1, PD2

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Galerie de données de validation

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  WB analysis of HepG2 using 15441-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15441-1-AP (PAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15441-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15441-1-AP (PAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15441-1-AP

  IP result of anti-PAF1 (IP:15441-1-AP, 3ug; Detection:15441-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of human ovary tumor using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below

Informations sur le produit

15441-1-AP cible PAF1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAF1 Protéine recombinante Ag7212
• Nom complet: Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC000017
• Symbole du gène: PAF1
• Identification du gène (NCBI): 54623
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAF1, also known as hPAF1 or PD2, is a PAF (RNA Polymerase II Associated Factor) complex subunit. Paf1 functions primarily to modulates transcript elongation. PAF1 occupies transcriptional enhancers and restrains hyperactivation of a subset of these enhancers(PMID: 28860207). The PAF complex also has a role in histone monoubiquitination. Paf1 is overexpressed in pancreatic cancer cells and has demonstrated potential oncogenic property. The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.

Publications

Species	Application	Title
human	WB	Autophagy; The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.; Authors - Liangde Zheng ; KD Validated
human	WB	J Cell Biol; Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.; Authors - Chen Chen
human	WB	Radiat Oncol; Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription.; Authors - Jing-Jie Zheng
human	WB	ACS Pharmacol Transl Sci; Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening; Authors - Lei Wang
human	WB	Sci Adv; CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation; Authors - Min Qiu
human	WB	Cell; SARS-CoV-2 variants evolve convergent strategies to remodel the host response; Authors - Mehdi Bouhaddou