- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-PABPC1,PABP
PABPC1,PABP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF/ICC, IP, RIP, ELISA
Conjugaison
Non conjugué
N° de cat : 10970-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HCT 116, cellules A549, cellules HeLa, cellules HT-29, cellules MCF-7, cellules NCI-H1299, cellules PC-3, tissu testiculaire de rat, tissu testiculaire de souris |
Résultats positifs en IP | tissu testiculaire de souris |
Résultats positifs en IHC | tissu pancréatique humain, tissu testiculaire humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules MCF-7 |
Dilution recommandée
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 3 publications below |
WB | See 19 publications below |
IHC | See 3 publications below |
IF | See 4 publications below |
IP | See 5 publications below |
RIP | See 1 publications below |
Informations sur le produit
10970-1-AP cible PABPC1,PABP dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | PABPC1,PABP Protéine recombinante Ag1422 |
Nom complet | poly(A) binding protein, cytoplasmic 1 |
Masse moléculaire calculée | 71 kDa |
Poids moléculaire observé | 71 kDa |
Numéro d’acquisition GenBank | BC015958 |
Symbole du gène | PABPC1 |
Identification du gène (NCBI) | 26986 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].
Protocole
Product Specific Protocols | |
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WB protocol for PABPC1,PABP antibody 10970-1-AP | Download protocol |
IHC protocol for PABPC1,PABP antibody 10970-1-AP | Download protocol |
IF protocol for PABPC1,PABP antibody 10970-1-AP | Download protocol |
IP protocol for PABPC1,PABP antibody 10970-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Exp Mol Med The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability | ||
Nat Commun An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis. | ||
J Exp Med E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non-small cell lung cancer. | ||
Clin Transl Med LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing | ||
Leukemia An atlas of bloodstream-accessible bone marrow proteins for site-directed therapy of acute myeloid leukemia. | ||
Haematologica YTHDF3 modulates hematopoietic stem cells by recognizing RNA m6A modification on Ccnd1.
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Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Elisa (Verified Customer) (01-13-2023) | PABPC1 (Poly(A) Binding Protein Cytoplasmic 1) staining (in magenta) and Hoechst (nuclear staining) in blue. PABPC1 ab shows a clear citoplasma staining. Method: RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 10'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).
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FH Zee (Verified Customer) (01-28-2020) | It worked very well when I performed western blot.
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FH George (Verified Customer) (09-11-2019) | Rb PABP Ig shows very little endogenous background staining with clear dispersed staining seen both in the nucleus and cytoplasm. 90mins heat shock at 42oC led to granular formation in the cytoplasm
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