• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-PABPC1,PABP

PABPC1,PABP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

N° de cat : 10970-1-AP

Synonymes

PABP, PABP1, PABPC1, PABPC 1, PABP-1



Applications testées

Résultats positifs en WBcellules HCT 116, cellules A549, cellules HeLa, cellules HT-29, cellules MCF-7, cellules NCI-H1299, cellules PC-3, tissu testiculaire de rat, tissu testiculaire de souris
Résultats positifs en IPtissu testiculaire de souris
Résultats positifs en IHCtissu pancréatique humain, tissu testiculaire humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules MCF-7

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10970-1-AP cible PABPC1,PABP dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PABPC1,PABP Protéine recombinante Ag1422
Nom complet poly(A) binding protein, cytoplasmic 1
Masse moléculaire calculée 71 kDa
Poids moléculaire observé 71 kDa
Numéro d’acquisition GenBankBC015958
Symbole du gène PABPC1
Identification du gène (NCBI) 26986
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].

Protocole

Product Specific Protocols
WB protocol for PABPC1,PABP antibody 10970-1-APDownload protocol
IHC protocol for PABPC1,PABP antibody 10970-1-APDownload protocol
IF protocol for PABPC1,PABP antibody 10970-1-APDownload protocol
IP protocol for PABPC1,PABP antibody 10970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Mol Med

The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability

Authors - Qianqian Yang
humanWB

Nat Commun

An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis.

Authors - Song Zhu
humanWB

J Exp Med

E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non-small cell lung cancer.

Authors - Ke Li
humanWB

Clin Transl Med

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing

Authors - Qi Wen Chen
ratIHC

Leukemia

An atlas of bloodstream-accessible bone marrow proteins for site-directed therapy of acute myeloid leukemia.

Authors - L Angenendt
mouseWB,IP

Haematologica

YTHDF3 modulates hematopoietic stem cells by recognizing RNA m6A modification on Ccnd1.

Authors - Xiaofei Zhang
  • KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elisa (Verified Customer) (01-13-2023)

PABPC1 (Poly(A) Binding Protein Cytoplasmic 1) staining (in magenta) and Hoechst (nuclear staining) in blue. PABPC1 ab shows a clear citoplasma staining. Method: RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 10'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)
PABPC1,PABP Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:10970-1-AP)
FH

Zee (Verified Customer) (01-28-2020)

It worked very well when I performed western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue
FH

George (Verified Customer) (09-11-2019)

Rb PABP Ig shows very little endogenous background staining with clear dispersed staining seen both in the nucleus and cytoplasm. 90mins heat shock at 42oC led to granular formation in the cytoplasm

  • Applications: Immunohistochemistry, Other,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Spinal Cord/Cortical Slice Culture