Anticorps Polyclonal de lapin anti-PA2G4

• Phare
• Validé par KD/KO

PA2G4 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15348-1-AP

Synonymes

EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4

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Galerie de données de validation

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  WB analysis of HEK-293 using 15348-1-AP

  WB result of PA2G4 antibody (15348-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PA2G4 transfected HEK-293 cells.

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  WB analysis using 15348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15348-1-AP (PA2G4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 15348-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15348-1-AP (PA2G4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15348-1-AP

  IP Result of anti-PA2G4 (IP:15348-1-AP, 4ug; Detection:15348-1-AP 1:1000) with Jurkat cells lysate 3000ug.

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  IHC staining of human breast cancer using 15348-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15348-1-AP (PA2G4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15348-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15348-1-AP (PA2G4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15348-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PA2G4 antibody 15348-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of MCF-7 using 15348-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug PA2G4 antibody (15348-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HEK-293, cellules HeLa, cellules HL-60, cellules Jurkat, cellules MCF-7, cellules PC-3, tissu splénique de rat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

15348-1-AP cible PA2G4 dans les applications de WB, RIP, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PA2G4 Protéine recombinante Ag7587
• Nom complet: proliferation-associated 2G4, 38kDa
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 42 kDa, 48 kDa
• Numéro d’acquisition GenBank: BC001951
• Symbole du gène: PA2G4
• Identification du gène (NCBI): 5036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a rabbit polyclonal antibody raised against full length PA2G4 of human origin.

Publications

Species	Application	Title
human	WB,IHC,IF,RIP	Cell Biosci; PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner.; Authors - Sheng Sun ; KD Validated
human	WB,IHC	FASEB J; Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain-of-function toxicity mechanism contributing to the pathogenesis of fragile X-associated premature ovarian insufficiency; Authors - Roseanne Rosario
		Methods Enzymol; Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells; Authors - Manuel Günnigmann
human	WB,IF,IP	J Dent Sci; Proliferation-associated 2G4 P48 is stabilized by malignant T-cell amplified sequence 1 and promotes the proliferation of head and neck squamous cell carcinoma; Authors - Legang Sun