Anticorps Polyclonal de lapin anti-DDX6

• Phare
• Validé par KD/KO

DDX6 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 14632-1-AP

Synonymes

ATP dependent RNA helicase p54, DDX6, DEAD box protein 6, HLR2, Oncogene RCK, P54, RCK

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Galerie de données de validation

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  WB analysis using 14632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14632-1-AP

  IP result of anti-DDX6 (IP:14632-1-AP, 4ug; Detection:14632-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human breast cancer using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 14632-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DDX6 antibody (14632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14632-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DDX6 antibody (14632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C2C12, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules hTERT-RPE1

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 5 publications below

Informations sur le produit

14632-1-AP cible DDX6 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX6 Protéine recombinante Ag6211
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC065007
• Symbole du gène: DDX6
• Identification du gène (NCBI): 1656
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P54, also named as DDX6, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. P54 is abundantly expressed in most tissues. In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas.

Publications

Species	Application	Title
mouse	WB,IF	J Genet Genomics; LSM14B coordinates protein component expression in the P-body and controls oocyte maturation; Authors - Huiru Zhang
human	WB,IF	iScience; The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.; Authors - Limei Zou
mouse	WB	Biol Reprod; Translational Activation of Developmental Messenger RNAs During Neonatal Mouse Testis Development.; Authors - Chappell Vesna A VA
human	WB	Biochem Biophys Res Commun; DDX6 is involved in the pathogenesis of inflammatory diseases via NF-κB activation; Authors - Seiichiro Naito ; KD Validated
mouse	IF	BMC Bioinformatics; SpotitPy: a semi-automated tool for object-based co-localization of fluorescent labels in microscopy images; Authors - Alexia Akalestou-Clocher
mouse	WB	Int J Legal Med; Candidate biomarkers in brown adipose tissue for post-mortem diagnosis of fatal hypothermia; Authors - Miao Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Roy (Verified Customer) (06-13-2024)

The antibody worked well to detect DDX6 in HeLa cells by WB (1/1000 dilution, Overnight incubation at 4°C) and by Immunofluorescence to observe P-Bodies (1h incubation at RT - 1/500).

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa