Anticorps Polyclonal de lapin anti-P4HA2

• Phare
• Validé par KD/KO

P4HA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 13759-1-AP

Synonymes

4 PH alpha 2, P4HA2

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Galerie de données de validation

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  WB analysis using 13759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13759-1-AP (P4HA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13759-1-AP

  WB result of P4HA2 antibody (13759-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P4HA2 transfected HeLa cells.

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  WB analysis of HeLa using 13759-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13759-1-AP (P4HA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13759-1-AP

  IP result of anti-P4HA2 (IP:13759-1-AP, 4ug; Detection:13759-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human cervical cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13759-1-AP (P4HA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13759-1-AP (P4HA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13759-1-AP (P4HA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13759-1-AP (P4HA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse ovary tissue using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP( LHCGR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary tissue using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP( LHCGR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 14 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Informations sur le produit

13759-1-AP cible P4HA2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P4HA2 Protéine recombinante Ag4714
• Nom complet: prolyl 4-hydroxylase, alpha polypeptide II
• Masse moléculaire calculée: 533 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC035813
• Symbole du gène: P4HA2
• Identification du gène (NCBI): 8974
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IHC,IF	Hepatology; P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases; Authors - Jun Zhang ; KD Validated
human	IHC	Blood; Prolyl 4-hydroxylase 2 promotes B-cell lymphoma progression via hydroxylation of Carabin.; Authors - Wei Jiang
		Nat Commun; Melanoblast transcriptome analysis reveals pathways promoting melanoma metastasis.; Authors - Kerrie L Marie
human	WB	EBioMedicine; Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.; Authors - Tianjiao Wang
human	WB	Cancer Res; Epigenetic SMAD3 repression in tumor-associated fibroblasts impairs fibrosis and response to the antifibrotic drug nintedanib in lung squamous cell carcinoma.; Authors - Rafael Ikemori
human	WB,IHC	Oncogene; Hepatitis B virus X protein promotes the development of liver fibrosis and hepatoma through downregulation of miR-30e targeting P4HA2 mRNA.; Authors - G X Feng