Anticorps Polyclonal de lapin anti-P15RS

• Phare
• Validé par KD/KO

P15RS Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 23652-1-AP

Synonymes

HsT3101, p15INK4B related protein, P15RS, RPRD1A

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Galerie de données de validation

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  WB analysis using 23652-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 23652-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23652-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 23652-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 23652-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 23652-1-AP (P15RS antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 23652-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using P15RS antibody (23652-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 23652-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P15RS antibody (23652-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules Jurkat, cellules L02, tissu de muscle squelettique de souris, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

23652-1-AP cible P15RS dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P15RS Protéine recombinante Ag20409
• Nom complet: regulation of nuclear pre-mRNA domain containing 1A
• Masse moléculaire calculée: 312 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC010136
• Symbole du gène: RPRD1A
• Identification du gène (NCBI): 55197
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPRD1A is upregulated in cells overexpressing cyclin-dependent kinase inhibitor p15(INK4b) and may have a role in cell cycle regulation. It contains a RAR domain that is involved in regulation of nuclear pre-mRNA, which suggests that P15RS may act as a nuclear regulation protein [PMID:12470661]. It also interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Besides, RPRD1A regulates cell cycle genes and attenuates WNT signaling [PMID: 20739273], and acts as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle [PMID: 22231121].

Publications

Species	Application	Title
human	WB	Mol Pharm; Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.; Authors - Xiaoman Chen
human	IP,WB,IHC,IF	Cell Death Dis; RPRD1A stabilizes NRF2 and aggravates HCC progression through competing with p62 for TRIM21 binding.; Authors - Xiaofan Feng ; KD Validated
human	WB	Mol Cell Biol; An Important Role for RPRD1B in the Heat Shock Response; Authors - Simona Cugusi
mouse	WB	Commun Biol; Circ-0075305 hinders gastric cancer stem cells by indirectly disrupting TCF4-β-catenin complex and downregulation of SOX9; Authors - Qi-Yue Chen