Anticorps Polyclonal de lapin anti-OXSR1

• Phare
• Validé par KD/KO

OXSR1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 15611-1-AP

Synonymes

KIAA1101, OSR1, oxidative stress responsive 1, OXSR1

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Galerie de données de validation

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  WB analysis using 15611-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15611-1-AP

  WB result of OXSR1 antibody (15611-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXSR1 transfected HeLa cells.

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  WB analysis of human liver using 15611-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15611-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15611-1-AP

  IP result of anti-OXSR1 (IP:15611-1-AP, 4ug; Detection:15611-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IP experiment of HEK-293 using 15611-1-AP

  IP result of anti-OXSR1 (IP:15611-1-AP, 4ug; Detection:15611-1-AP 1:300) with HEK-293 cells lysate 2320 ug.

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  IHC staining of human prostate cancer using 15611-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15611-1-AP (OXSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15611-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15611-1-AP (OXSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15611-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OXSR1 antibody (15611-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules U-251, tissu hépatique humain, tissu testiculaire humain
• Résultats positifs en IP: cellules HeLa, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15611-1-AP cible OXSR1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OXSR1 Protéine recombinante Ag8002
• Nom complet: oxidative-stress responsive 1
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC008726
• Symbole du gène: OXSR1
• Identification du gène (NCBI): 9943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Oxidative-stress responsive 1(OXSR1) is also named as KIAA1101, OSR1 and belongs to the STE Ser/Thr protein kinase family. It contains an N-terminal Ste20-like ser/thr kinase domain and 2 C-terminal regions, which has a putative caspase-3 cleavage site at the end. OXSR1's interaction with WNK1 is required for NKCC function, and it modulates the G protein sensitivity of PAK by phosphorylation of PAK1.Western blot analysis detected Oxsr1 at an apparent molecular mass of 58 kD in all mouse tissues examined except thymus. Cell fractionation and immunofluorescence analysis of HeLa cells showed that OXSR1 was distributed throughout the cell and OXSR1 could phosphorylate a test substrate and itself(PMID:14707132).

Publications

Species	Application	Title
human	IHC,WB	Bioengineered; Upregulation of Oxidative stress-responsive 1(OXSR1) Predicts Poor Prognosis and Promotes Hepatocellular Carcinoma Progression.; Authors - Jianhui Chen
rat	WB,IF	Adv Sci (Weinh); Modulation of Cerebrospinal Fluid Dysregulation via a SPAK and OSR1 Targeted Framework Nucleic Acid in Hydrocephalus; Authors - Qiguang Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jennifer (Verified Customer) (03-23-2022)

Image provided from optimisation plate for OXSR1 for rat brain lysates. Analysed using Simple Western Analysis (WES) by Protein Simple. Finalised antibody and protein concentration for samples produced a single band with no non-specific binding.

• Applications: Other
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Rat and mouse brain lysate

OXSR1 Antibody Other validation (1:100 dilution) in Rat and mouse brain lysate (Cat no:15611-1-AP)