Anticorps Polyclonal de lapin anti-OXR1

OXR1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA, IF

Conjugaison

Non conjugué

Publications

7

N° de cat : 13514-1-AP

Synonymes

Nbla00307, oxidation resistance 1, Oxidation resistance protein 1, OXR1

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Galerie de données de validation

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  WB analysis using 13514-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13514-1-AP (OXR1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13514-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13514-1-AP (OXR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 13514-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13514-1-AP (OXR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13514-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13514-1-AP (OXR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 13514-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13514-1-AP (OXR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules Neuro-2a
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

13514-1-AP cible OXR1 dans les applications de WB, IHC, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OXR1 Protéine recombinante Ag4439
• Nom complet: oxidation resistance 1
• Masse moléculaire calculée: 758 aa, 85 kDa
• Poids moléculaire observé: 120-140 kDa
• Numéro d’acquisition GenBank: BC032710
• Symbole du gène: OXR1
• Identification du gène (NCBI): 55074
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Oxidation resistance protein 1 (OXR1) belongs to the OXR1 family. The major function of OXR1 is to control the expression of genes that alleviate oxidative stress by increasing cellular resistance to reactive oxygen species (ROS) and the stress these molecules cause the cell. OXR1's ability to reduce oxidative stress and neurodegeneration in multiple diseases strongly suggests that it can be an effective therapeutic target (PMID: 33384581).Biochemical experiments show that OXR1 inhibits V1-ATPase and causes disassembly of the holoenzyme, suggesting that OXR1 plays a direct role in V-ATPase regulation (PMID: 34918374). OXR1 has 8 isoforms with the molecular mass of 25, 28, 34, 56, 94, 95 and 98 kDa. Sometimes higher molecular weight around 120-140 kDa can also be observed, which may be a modified variant of OXR1.

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
mouse	WB	J Inflamm Res; Orexin-A Attenuates Inflammatory Responses in Lipopolysaccharide-Induced Neural Stem Cells by Regulating NF-KB and Phosphorylation of MAPK/P38/Erk Pathways.; Authors - Wen Ye
rat	WB	Front Mol Biosci; 6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis.; Authors - Jing Liu
mouse	WB	Brain Res Bull; Aerobic training associated with an active lifestyle exerts a protective effect against oxidative damage in hypothalamus and liver: The involvement of energy metabolism.; Authors - Pedro P M Scariot
mouse	WB	Redox Biol; Iridium metal complex targeting oxidation resistance 1 protein attenuates spinal cord injury by inhibiting oxidative stress-associated reactive oxygen species; Authors - Cheng Peng
mouse	WB	Antioxidants (Basel); Neuroinflammation and Lysosomal Abnormalities Characterise the Essential Role for Oxidation Resistance 1 in the Developing and Adult Cerebellum; Authors - Eboni M V Bucknor ; KO Validated